Vaping e-cigarettes with high amounts of nicotine appears to impact how often and how heavily teens smoke and vape in the future, a new study finds.

In 2016, an estimated 11 percent of U.S. high school students used e-cigarettes. Past research has found that that teen vaping can lead to smoking (SN: 9/19/15, p. 14). The new study, published online October 23 in JAMA Pediatrics, is the first look at whether vaping higher amounts of nicotine is associated with more frequent and more intense vaping and cigarette use in the future.
Researchers at the University of Southern California surveyed 181 10th-graders from 10 high schools in the Los Angeles area who had reported vaping in the previous 30 days, then followed up six months later, when the students were 11th-graders. The teens answered questions about how much and how often they had smoked and vaped in the past 30 days and about the amount of nicotine in their vaping liquid. The researchers categorized the amount of nicotine as none, low (up to 5 milligrams per milliliter), medium (6 to 17 mg/mL) or high (18 mg/mL or more).

With each step up in nicotine concentration, teens were about twice as likely to report frequent smoking versus no smoking at the six-month follow-up. Teens who vaped a high-nicotine liquid smoked seven times as many cigarettes per day as those who vaped without nicotine.

Also with each nicotine level increase, teens were about 1½ times as likely to report frequent vaping than no vaping at all. Vaping high-nicotine liquid led to almost 2½ times as many episodes of vaping per day compared with no-nicotine vaping, and kids took more puffs each time they vaped.

“This study is important because it begins to chip away at the ‘black box’ that links e-cigarette use with later use of regular cigarettes,” says sociologist Richard Miech of the University of Michigan in Ann Arbor. “Ideally, studies like this will encourage government agencies to develop policies that will make it very difficult for youth to obtain e-liquids with nicotine.”
In 2016, then-U.S. Surgeon General Vivek Murthy released a report on e-cigarettes, concluding that using nicotine-containing products in any form is not safe for youth. Studies find an association between nicotine use in teens and problems with learning, attention and impulse control, as well as addiction (SN: 7/11/15, p. 18).

A soft heart keeps Enceladus warm from the inside. Friction within its porous core could help Saturn’s icy moon maintain a liquid ocean for billions of years and explain why it sprays plumes from its south pole, astronomers report November 6 in Nature Astronomy.

Observations in 2015 showed that Enceladus’ icy surface is a shell that’s completely detached from its rocky core, meaning the ocean spans the entire globe (SN: 10/17/15, p. 8). Those measurements also showed that the ice is not thick enough to keep the ocean liquid.
Other icy moons, like Jupiter’s Europa, keep subsurface oceans warm through the energy generated by gravitational flexing of the ice itself. But if that were Enceladus’ only heat source, its ocean would have frozen within 30 million years, a fraction of the age of the solar system, which formed roughly 4.6 billion years ago.

Planetary scientist Gaël Choblet of the University of Nantes in France and his colleagues tested whether friction in the sand and gravel thought to make up Enceladus’ core could heat things up.

The team made computer simulations of water circulating through the spongy core using data from the Cassini spacecraft and geoengineering experiments with sand and gravel on Earth. They found that, depending on the core’s makeup, the ocean should get enough heat to stay liquid for tens of millions to billions of years.

The simulations also showed that certain hot spots in the core, including at the poles, correspond to regions where the ice shell is thinner.
“That was quite cool,” Choblet says. “It explains the internal structure and the way things are organized and the dynamics interior to Enceladus.”

And that could explain why the moon spews plumes of water from its south pole: More heat from the core at that spot could melt the ice and let water out. It doesn’t explain why the north pole is plume-free, though.

Recent reports of African and North American animal fossils bearing stone-tool marks from being butchered a remarkably long time ago may be a crock. Make that a croc.

Crocodile bites damage animal bones in virtually the same ways that stone tools do, say paleoanthropologist Yonatan Sahle of the University of Tübingen in Germany and his colleagues. Animal bones allegedly cut up for meat around 3.4 million years ago in East Africa (SN: 9/11/10, p. 8) and around 130,000 years ago in what’s now California (SN: 5/27/17, p. 7) come from lakeside and coastal areas. Those are places where crocodiles could have wreaked damage now mistaken for butchery, the scientists report online the week of November 6 in the Proceedings of the National Academy of Sciences.
Larger samples of animal fossils, including complete bones from various parts of the body, are needed to begin to tease apart the types of damage caused by stone tools, crocodile bites and trampling of bones by living animals, Sahle’s team concludes. “More experimental work on bone damage caused by big, hungry crocs is also critical,” says coauthor Tim White, a paleoanthropologist at the University of California, Berkeley.

In a field where researchers reap big rewards for publishing media-grabbing results in high-profile journals, such evidence could rein in temptations to over-interpret results, says archaeologist David Braun of George Washington University in Washington, D.C., who did not participate in the new study or the two earlier ones. “There’s a push to publish extraordinary findings, but evolutionary researchers always have to weigh what’s interesting versus what’s correct.”

Authors of the ancient butchery papers agree that bone marks made by crocodiles deserve closer study and careful comparison with proposed stone-tool marks. But the researchers stand their ground on their original conclusions.

Microscopic investigations in the 1980s led some researchers to conclude that carnivores such as hyenas leave U-shaped marks on bones. In contrast, they argued, stone tools leave V-shaped incisions with internal ridges. And hammering stones create signature pits and striations.
Sahle’s group expanded on research previously conducted by paleoanthropologist Jackson Njau of Indiana University Bloomington. In his 2006 doctoral dissertation, Njau reported that bone damage produced by feeding crocodiles looks much like stone-tool incisions and pits, with a few distinctive twists such as deep scratches. Njau retrieved and studied cow and goat bones from carcasses that had been eaten by crocodiles housed at two animal farms in Tanzania.

In the new study, the scientists used Njau’s findings to reassess marks on fossils previously excavated in Ethiopia and dating to around 4.2 million, 3.4 million and 2.5 million years ago. Damage to these fossils has generally been attributed to butchery with stone tools.

Incisions and pits on arm bones from an ancient hominid, Australopithecus anamensis, and similar marks on a horse’s leg bone likely resulted from crocodile bites and not stone-tool use, as initially suspected, the investigators say. If stone tools had indeed damaged the A. anamensis remains, that would raise the possibility of cannibalism — a difficult behavior to confirm with fossils. Tellingly, Sahle’s team argues, these bones come from what were once waterside areas. Some were found in the same sediment layer as crocodile remains. Marks on these bones include deep scratches consistent with crocodile bites.

The horse fossil comes from a spot along an ancient lakeshore where no stone tools have been found, a further clue in favor of damage from croc bites.

Jagged pits, incisions and other marks scar a leg fragment and lower jaw from an ancient hoofed animal. But microscopic analyses could not definitively attribute the damage to stone tools or crocodile bites.

In light of these findings, the ancient California and 3.4-million-year-old East Africa bones should also be reexamined with the possibility of croc damage in mind, White says. For now, the earliest confirmed stone-tool marks occur on animal bones from two East African sites dating to around 2.5 million years ago (SN: 4/17/04, p. 254), he adds.

The range of crocodile marks described in the new study doesn’t look “especially like” damage to the 130,000-year-old mastodon bones on California’s coast, says paleontologist Daniel Fisher of the University of Michigan in Ann Arbor, a coauthor of the ancient California bones paper. No fossil evidence indicates crocodiles lived there at that time, he adds. Several lines of evidence, including pounding marks and damage near joints, point to stone-tool use at the West Coast site, says archaeologist Richard Fullagar of the University of Wollongong in Australia, also a coauthor of the mastodon paper.

Further studies of the 3.4-million-year-old African bones previously reported as probable examples of animal butchery will statistically compare the probability of various causes for particular marks, including crocodile bites, says Shannon McPherron, the lead author of the earlier study and an archaeologist at the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany. In that way, researchers can assess whether any one cause stands out as the strongest candidate.

Two days before plunging into Saturn, the Cassini spacecraft took one last look around the planet it had orbited for more than 13 years.

The view of Saturn above, released November 21, is actually made from 42 images that have been stitched together. Six moons — Enceladus, Epimetheus, Janus, Mimas, Pandora and Prometheus — are faintly visible as dots surrounding the gas giant (see the annotated image below). Cassini was about 1.1 million kilometers away from Saturn when it took the images on September 13. The whole observation took a little over two hours.

On September 11, Cassini set itself on a collision course with Saturn, and on September 15, the probe ended its mission by burning up in Saturn’s atmosphere, taking data all the way down.

MINNEAPOLIS — Pesticides that kill insects can also have short-term effects on seed-eating birds. Ingesting even small amounts of imidacloprid, a common neonicotinoid pesticide, can disorient migratory white-crowned sparrows, researchers report.

Neonicotinoid pesticides were designed to be safer than traditional pesticides: toxic to insects, but comparatively harmless to other animals. But the new findings add to evidence suggesting that the widely used pesticides, which are chemically similar to nicotine, might be sending ecological ripples beyond the intended targets.
In lab studies, researchers captured wild white-crowned sparrows, Zonotrichia leucophrys, that were migrating north and fed them small doses of imidacloprid for three days — the amount that birds would get from eating a few pesticide-coated wheat seeds. The birds that ate the pesticides lost weight, study coauthor Margaret Eng reported November 15 at the annual meeting of the Society of Environmental Toxicology and Chemistry North America.

And when placed in a large, inverted funnel used to study birds’ migratory orientations, the neonic-fed birds tried to fly in directions other than north. Birds that consumed sunflower oil instead showed no ill effects.

For the birds that ate pesticides, the damage was temporary — after two weeks, the birds regained normal function and body weight, Eng, a toxicologist at the University of Saskatchewan in Saskatoon, Canada, and her colleagues also reported November 9 in Scientific Reports.

The fact that the effects reverse after a period of time is “good news,” says Thomas Bean, a toxicologist at the University of Maryland in College Park who wasn’t part of the study. The short-term malaise might incentivize birds to avoid that food in the future. Bean has found that Japanese quail, Coturnix japonica, also show similar temporary behavioral effects in response to neonicotinoids.
Preliminary results from field studies also appear to confirm the published lab findings. Eng’s team outfitted white-crowned sparrows in the wild with tiny tracking tags. The scientists gave the birds small amounts of pesticides, held the birds for six hours, and then released them.

When released, the birds still had traces of the chemicals in their blood plasma, the researchers reported at the November meeting. On average, there wasn’t a difference between groups in how long the birds hung around before resuming migration, but all of the birds that waited an abnormally long time had eaten neonics. Those animals’ flight paths also appeared to be slightly skewed from the route favored by the control birds.

Those analyses are preliminary, cautions Eng, and a closer look at the data could change the story.

A skull and other fossils from northeastern Australia belong to a new species in the extinct family of marsupial lions.

This newly named species, Wakaleo schouteni, was a predator about the size of a border collie, says vertebrate paleontologist Anna Gillespie of the University of New South Wales in Sydney. At least 18 million years ago (and perhaps as early as 23 million years ago), it roamed what were then hot, humid forests. Its sturdy forelimbs suggest it could chase possums, lizards and other small prey up into trees. Gillespie expects W. shouteni — the 10th species named in its family — carried its young in a pouch as kangaroos, koalas and other marsupials do.
Actual lions evolved on a different fork in the mammal genealogical tree, but Australia’s marsupial lions got their feline nickname from the size and slicing teeth of the first species named, in 1859. Thylacoleo carnifex was about as big as a lion. And its formidable teeth could cut flesh. But unlike other pointy-toothed predators, marsupial lions evolved a horizontal cutting edge. A bottom tooth stretched back along the jawline on each side, its slicer edge as long as four regular teeth. An upper tooth extended too, giving this marsupial lion a bite like a “bolt cutter,” Gillespie says.

The newly identified species lived some 17 million years before its big bolt-cutter relative. Though the new species’ tooth number matched those of typical early marsupials, W. schouteni already had a somewhat elongated tooth just in front of the molars, Gillespie and colleagues report December 7 in the Journal of Systematic Paleontology. W. schouteni is “pushing the history of marsupial lions deeper into time,” she says.

Old blood can prematurely age the brains of young mice, and scientists may now be closer to understanding how. A protein located in the cells that form a barrier between the brain and blood could be partly to blame, experiments on mice suggest.

If something similar happens in humans, scientists say, methods for countering the protein may hold promise for treating age-related brain decline.

The preliminary study, published online January 3 at bioRxiv.org, focused on a form of the protein known as VCAM1, which interacts with immune cells in response to inflammation. As mice and humans age, levels of that protein circulating in the blood rise, Alzheimer researcher Tony Wyss-Coray at Stanford University and colleagues found.
After injecting young mice behind an eye with plasma from old mice, the team discovered that VCAM1 levels also rose in certain parts of the blood-brain barrier, a mesh of tightly woven cells that protect the brain from harmful factors in the blood. The young mice showed signs of brain deterioration as well, including inflammation and decreased birthrates of new nerve cells. Plasma from young mice had no such effects.

Interfering with VCAM1 may help prevent the premature aging of brains. Plasma from old mice didn’t have a strong effect when injected into young mice genetically engineered to lack VCAM1 in certain blood-brain barrier cells. Nor did it affect mice treated with antibodies that blocked the activity of VCAM1. Those antibodies also seemed to help the brains of older mice that had aged naturally, the team found.

The results suggest that anti-aging treatments targeting specific aspects of the blood-brain barrier may hold promise.

Wikipedia: The settler of dinnertime disputes and the savior of those who cheat on trivia night. Quick, what country has the Nile’s headwaters? What year did Gershwin write “Rhapsody in Blue”? Wikipedia has the answer to all your burning trivia questions — including ones about science.

With hundreds of thousands of scientific entries, Wikipedia offers a quick reference for the molecular formula of Zoloft, who the inventor of the 3-D printer is and the fact that the theory of plate tectonics is only about 100 years old. The website is a gold mine for science fans, science bloggers and scientists alike. But even though scientists use Wikipedia, they don’t tend to admit it. The site rarely ends up in a paper’s citations as the source of, say, the history of the gut-brain axis or the chemical formula for polyvinyl chloride.
But scientists are browsing Wikipedia just like everyone else. A recent analysis found that Wikipedia stays up-to-date on the latest research — and vocabulary from those Wikipedia articles finds its way into scientific papers. The results don’t just reveal the Wiki-habits of the ivory tower. They also show that the free, widely available information source is playing a role in research progress, especially in poorer countries.

Teachers in middle school, high school and college drill it in to their students: Wikipedia is not a citable source. Anyone can edit Wikipedia, and articles can change from day to day — sometimes by as little as a comma, other times being completely rewritten overnight. “[Wikipedia] has a reputation for being untrustworthy,” says Thomas Shafee, a biochemist at La Trobe University in Melbourne, Australia.

But those same teachers — even the college professors — who warn students away from Wikipedia are using the site themselves. “Academics use Wikipedia all the time because we’re human. It’s something everyone is doing,” says Doug Hanley, a macroeconomist at the University of Pittsburgh.

And the site’s unreliable reputation may be unwarranted. Wikipedia is not any less consistent than Encyclopedia Britannica, a 2005 Nature study showed (a conclusion that the encyclopedia itself vehemently objected to). Citing it as a source, however, is still a bridge too far. “It’s not respected like academic resources,” Shafee notes.
Academic science may not respect Wikipedia, but Wikipedia certainly loves science. Of the roughly 5.5 million articles, half a million to a million of them touch on scientific topics. And constant additions from hundreds of thousands of editors mean that entries can be very up to date on the latest scientific literature.

How recently published findings affect Wikipedia is easy to track. They’re cited on Wikipedia, after all. But does the relationship go the other way? Do scientific posts on Wikipedia worm their way into the academic literature, even though they are never cited? Hanley and his colleague Neil Thompson, an innovation scholar at MIT, decided to approach the question on two fronts.

First, they determined the 1.1 million most common scientific words in published articles from the scientific publishing giant Elsevier. Then, Hanley and Thompson examined how often those same words were added to or deleted from Wikipedia over time, and cited in the research literature. The researchers focused on two fields, chemistry and econometrics — a new area that develops statistical tests for economics.

There was a clear connection between the language in scientific papers and the language on Wikipedia. “Some new topic comes up and it gets exciting, it will generate a new Wikipedia page,” Thompson notes. The language on that new page was then connected to later scientific work. After a new entry was published, Hanley and Thompson showed, later scientific papers contained more language similar to the Wikipedia article than to papers in the field published before the new Wikipedia entry. There was a definite association between the language in the Wikipedia article and future scientific papers.

But was Wikipedia itself the source of that language? This part of the study can’t answer that. It only observes words increasing together in two different spaces. It can’t prove that scientists were reading Wikipedia and using it in their work.

So the researchers created new Wikipedia articles from scratch to find out if the language in them affected the scientific literature in return. Hanley and Thompson had graduate students in chemistry and in econometrics write up new Wikipedia articles on topics that weren’t yet on the site. The students wrote 43 chemistry articles and 45 econometrics articles. Then, half of the articles in each set got published to Wikipedia in January 2015, and the other half were held back as controls. The researchers gave the articles three months to percolate through the internet. Then they examined the next six months’ worth of published scientific papers in those fields for specific language used in the published Wikipedia entries, and compared it to the language in the entries that never got published.

In chemistry, at least, the new topics proved popular. Both the published and control Wikipedia page entries had been selected from graduate level topics in chemistry that weren’t yet covered on Wikipedia. They included entries such as the synthesis of hydrastine (the precursor to a drug that stops bleeding). People were interested enough to view the new articles on average 4,400 times per month.

The articles’ words trickled into to the scientific literature. In the six months after publishing, the entries influenced about 1 in 300 words in the newly published papers in that chemical discipline. And scientific papers on a topic covered in Wikipedia became slightly more like the Wikipedia article over time. For example, if chemists wrote about the synthesis of hydrastine — one of the new Wikipedia articles — published scientific papers more often used phrases like “Passarini reaction,” a term used in the Wikipedia entry. But if an article never went on to Wikipedia, the scientific papers published on the topic didn’t become any more similar to the never-published article (which could have happened if the topics were merely getting more popular). Hanley and Thompson published a preprint of their work to the Social Science Research Network on September 26.

Unfortunately, there was no number of Wikipedia articles that could make econometrics happen. “We wanted something on the edge of a discipline,” Thompson says. But it was a little too edgy. The new Wikipedia entries in that field got one-thirtieth of the views that chemistry articles did. Thompson and Hanley couldn’t get enough data from the articles to make any conclusions at all. Better luck next time, econometrics.

The relationship between Wikipedia entries and the scientific literature wasn’t the same in all regions. When Hanley and Thompson broke the published scientific papers down by the gross domestic product of their countries of origin, they found that Wikipedia articles had a stronger effect on the vocabulary in scientific papers published by scientists in countries with weaker economies. “If you think about it, if you’re a relatively rich country, you have access at your institution to a whole list of journals and the underlying scientific literature,” Hanley notes. Institutions in poorer countries, however, may not be able to afford expensive journal subscriptions, so scientists in those countries may rely more heavily on publicly available sources like Wikipedia.

The Wikipedia study is “excellent research design and very solid analysis,” says Heather Ford, who studies digital politics at the University of Leeds in England. “As far as I know, this is the first paper that attributes a strong link between what is on Wikipedia and the development of science.” But, she says, this is only within chemistry. The influence may be different in different fields.

“It’s addressing a question long in people’s minds but difficult to pin down and prove,” says Shafee. It’s a link, but tracking language, he explains, isn’t the same as finding out how ideas and concepts were moving from Wikipedia into the ivory tower. “It’s a real cliché to say more research is needed, but I think in this case it’s probably true.”

Hanley and Thompson would be the first to agree. “I think about this as a first step,” Hanley says. “It’s showing that Wikipedia is not just a passive resource, it also has an effect on the frontiers of knowledge.”

It’s a good reason for scientists get in and edit entries within their expertise, Thompson notes. “This is a big resource for science and I think we need to recognize that,” Thompson says. “There’s value in making sure the science on Wikipedia is as good and complete as possible.” Good scientific entries might not just settle arguments. They might also help science advance. After all, scientists are watching, even if they won’t admit it.

These fins were made for walking, and that’s just what these fish do — thanks to wiring that evolved long before vertebrates set foot on land.

Little skates use two footlike fins on their undersides to move along the ocean floor. With an alternating left-right stride powered by muscles flexing and extending, the movement of these fish looks a lot like that of many land-based animals.

Now, genetic tests show why: Little skates and land vertebrates share the same genetic blueprint for development of the nerve cells needed for limb movement, researchers report online February 8 in Cell. This work is the first to look at the origins of the neural circuitry needed for walking, the authors say.
“This is fantastically interesting natural history,” says Ted Daeschler, a vertebrate paleontologist at the Academy of Natural Sciences in Philadelphia.

“Neurons essential for us to walk originated in ancient fish species,” says Jeremy Dasen, a neuroscientist at New York University. Based on fossil records, Dasen’s team estimates that the common ancestor of all land vertebrates and skates lived around 420 million years ago — perhaps tens of millions of years before vertebrates moved onto land (SN: 1/14/12, p. 12).
Little skates (Leucoraja erinacea) belong to an evolutionarily primitive group. Skates haven’t changed much since their ancestors split from the fish that evolved into land-rovers, so finding the same neural circuitry in skates and land vertebrates was surprising.

The path to discovery started when Dasen and coauthor Heekyung Jung, now at Stanford University, saw YouTube videos of the little skates walking.

“I was completely flabbergasted,” Dasen says. “I knew some species of fish could walk, but I didn’t know about these.”

Most fish swim by undulating their bodies and tails, but little skates have a spine that remains relatively straight. Instead, little skates move by flapping pancake-shaped pectoral fins and walking on “feet,” two fins tucked along the pelvis.

Measurements of the little skates’ movements found that they were “strikingly similar” to bipedal walking, says Jung, who did the work while at NYU. To investigate how that similarity arose, the researchers looked to motor nerve cells, which are responsible for controlling muscles. Each kind of movement requires different kinds of motor nerve cells, Dasen says.

The building of that neural circuitry is controlled in part by Hox genes, which help set the body plan, where limbs and muscles and nerves should go. For instance, snakes and other animals that have lost some Hox genes have bodies that move in the slinky, slithery undulations that many fish use to swim underwater.

By comparing Hox genes in L. erinacea and mice, researchers discovered that both have Hox6/7 and Hox10 genes and that these genes have similar roles in both. Hox6/7 is important for the development of the neural circuitry used to move the skates’ pectoral fins and the mice’s front legs; Hox10 plays the same role for the footlike fins in little skates and hind limbs in mice. Other genes and neural circuitry for motor control were also conserved, or unchanged, between little skates and mice. The findings suggest that both skates and mice share a common ancestor with similar genetics for locomotion.

The takeaway is that “vertebrates are all very similar to each other,” says Daeschler. “Evolution works by tinkering. We’re all using what we inherited — a tinkered version of circuitry that began 400-plus million years ago.”

Orangutan numbers on the Southeast Asian island of Borneo plummeted from 1999 to 2015, more as a result of human hunting than habitat loss, an international research team finds.

Over those 16 years, Borneo’s orangutan population declined by about 148,500 individuals. A majority of those losses occurred in the intact or selectively logged forests where most orangutans live, primatologist Maria Voigt of the Max Planck Institute for Evolutionary Anthropology in Leipzig, Germany, and colleagues report February 15 in Current Biology.
“Orangutan killing is likely the number one threat to orangutans,” says study coauthor Serge Wich, a biologist and ecologist at Liverpool John Moores University in England. Humans hunt the forest-dwelling apes for food, or to prevent them from raiding crops, the investigators say. People also kill adult orangutans to steal their babies for the international pet trade.

Between 70,000 and roughly 100,000 orangutans currently live on Borneo, Wich says. That’s substantially higher than previous population estimates. The new figures are based on the most extensive survey to date, using ground and air monitoring of orangutans’ tree nests. Orangutans live only on Borneo and the island of Sumatra and are endangered in both places.

Still, smaller orangutan populations in deforested areas of Borneo — due to logging or conversion to farm land — experienced the severest rates of decline, up to a 75 percent drop in one region.

Satellite data indicate that Borneo’s forest area has already declined by about 30 percent from 1973 to 2010. In the next 35 years, Voigt’s team calculates that further habitat destruction alone will lead to the loss of around 45,000 more of these apes. “Add hunting to that and it’s a lethal mix,” Wich says. But small groups of Bornean orangutans living in protected zones and selectively logged areas will likely avoid extinction, the researchers say.